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- Sep 06, 2013 · laboratories, including the Lowry, the Coomassie blue, the A 280, and the Warburg-Christian methods. Each of these methods has strengths and weaknesses. The A 280 method takes advantage of the absorbance of light at 280 nm by the amino acids tyrosine and tryptophan. The general method is just to take a solution of your protein, stick it
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